RNA Polymerase III Antibody, IgG
Also known as: RNA POL 3
Use
The presence of RNA polymerase III IgG antibody, when considered in conjunction with other laboratory and clinical findings, is an aid in the diagnosis of systemic sclerosis (SSc) with increased incidence of skin involvement and renal crisis with the diffuse cutaneous form of SSc. RNA polymerase III IgG antibodies occur in about 11-23 percent of SSc patients and typically in the absence of anti-centromere and anti-Scl-70 antibodies.
Special Instructions
First-line test for the evaluation of systemic sclerosis, or connective tissue disease with renal or cutaneous involvement. Preferred test is the Comprehensive Systemic Sclerosis Panel (3000480).
Limitations
A negative result indicates no detectable IgG antibodies to the dominant antigen of RNA polymerase III and does not rule out the possibility of SSc. False-positive results may occur due to non-specific binding of immune complexes. Strong clinical correlation is recommended. If clinical suspicion remains, consider additional testing for other antibodies associated with SSc, including centromere, Scl-70, U3-RNP, PM/Scl, or Th/To.
New York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 79182-2
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.2 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Separate serum from cells ASAP or within 2 hours of collection. Transfer serum to an ARUP Standard Transport Tube.
Storage Instructions
Refrigerated.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year |