Wilms Tumor (WT-1), N-terminus by Immunohistochemistry
Also known as: WT1 IHC
Use
Immunohistochemical stain for the N‑terminus of Wilms Tumor 1 (WT‑1) protein, useful as a technical service (stain‑and‑return) to assist in diagnosing entities such as Wilms tumor and distinguishing tumor types based on nuclear protein expression patterns.
Special Instructions
Technical service only (“stain and return”). Electronic request recommended. If electronic ordering is unavailable, use ARUP Immunohistochemistry Stain Form (#32978) with ARUP client number. Refer to ARUP Immunohistochemistry Stain Offerings brochure.
Limitations
Only applicable to tissue or cellblock specimens; non‑representative tissue types or depleted specimens are unacceptable. Precut slides should not be oven‑baked. Frozen specimens unacceptable. Interpretation limited to technical staining; final interpretation requires pathologist.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue block or ≥5 unstained (3‑ to 5‑micron) positively charged slides
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock); transport tissue block or 5 unstained positively charged slides (min: 2 slides) in tissue transport kit ARUP #47808; if precut slides, do not oven bake.
Storage Instructions
Protect from excessive heat; transport at room temperature or refrigerated; ship in cooled container during summer.
Causes for Rejection
Non‑representative tissue type; depleted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |