Alkaline Phosphatase
Use
Causes of high alkaline phosphatase include bone growth, healing fracture, acromegaly, osteogenic sarcoma, liver or bone metastases, leukemia, myelofibrosis, and rarely myeloma. Alkaline phosphatase is used as a tumor marker.3,4
Special Instructions
Values obtained with different assay methods should not be used interchangeably in serial testing. This test is intended for one-time use only. For serial monitoring, use test number 480004. Ensure serum or plasma is separated from cells within 45 minutes of collection.
Limitations
Used alone, alkaline phosphatase may be misleading as it can reflect a range of physiological or pathological conditions. Different assay methods provide non-interchangeable values, necessitating consistent use of a single assay method for monitoring purposes. The test is effective in confirming biliary abnormalities alongside GT, as GT is elevated in hepatobiliary disease, not in uncomplicated bone disease.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 6768-6
- 6768-6
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.7 mL
Container
Red-top tube, gel-barrier tube, or green-top (lithium heparin) tube; do not use oxalate, EDTA or citrate plasma
Collection Instructions
Separate serum or plasma from cells within 45 minutes of collection. If a red-top tube or green-top tube is used, transfer separated serum or plasma to a plastic transport tube.
Storage Instructions
Room temperature
Causes for Rejection
Hemolysis; plasma specimen; specimen collected in EDTA tube; improper labeling
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |