Anti-Scl-70 Ab (RDL)
Also known as: SCLER 70, topoisomerase 1
Use
The presence of anti-Scl-70 antibodies supports the diagnosis of scleroderma but does not exclude additional diagnosis, e.g., scleroderma and SLE or scleroderma and Sjogren's syndrome. Anti-Scl-70 antibodies are present in 20% of scleroderma patients irrespective of age and in the same percentage of males and females. In American patients with proximal scleroderma, anti-Scl-70 Abs are more common in African Americans than in Caucasians.
Special Instructions
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Limitations
The detection of antibodies does not exclude other associated autoimmune conditions. A negative result does not rule out scleroderma, as anti-Scl-70 antibodies are not present in all cases. Further confirmatory tests might be required for a conclusive diagnosis.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 5348-8
- 5348-8
Result Turnaround Time
14-21 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.
Storage Instructions
Refrigerate or freeze.
Causes for Rejection
Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 60 days |