Anti-Smooth Muscle Antibodies by Indirect Fluorescent Antibody (IFA) (RDL)
Use
Smooth muscle antibodies (SMA) are the standard diagnostic marker of autoimmune hepatitis (AH).
Special Instructions
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Limitations
The test uses indirect fluorescent antibody (IFA) methodology, which may have variability in interpreting fluorescence intensity. Non-specific binding can lead to false-positive results. External factors such as hemolysis, bacterial contamination, lipemia, or icterus in specimens can affect the results. The test is specifically for serum and cannot be performed on other types of specimens.
Methodology
Immunoassay (Indirect fluorescent antibody (IFA))
Biomarkers
LOINC Codes
- 5358-7
- 26971-2
Result Turnaround Time
7-14 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.
Storage Instructions
Refrigerate or freeze.
Causes for Rejection
Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 60 days |