Auto-Crossmatch
Also known as: Flow Crossmatch, HLA Crossmatch, T-Cell and B-Cell Crossmatch
Use
Detection of the presence of autoantibodies (HLA) in a solid organ recipient. T- and B-cell crossmatch detects the presence of anti-Class I/Class II HLA antibodies in the patient.
Special Instructions
To schedule this test, you must telephone 800-533-1037, HLA customer service. Testing schedules may vary, so it is recommended to confirm the schedule ahead of the sample collection.
Limitations
This test primarily detects antibodies to HLA, but other non-HLA specific antibodies could also lead to positive T- and/or B-cell crossmatches. It only demonstrates antibodies that recognize donor antigens and cannot differentiate between clinically relevant antibodies, such as those that bind complement, and those without clinical significance. The assay detects IgG antidonor antibodies but not IgM or other immunoglobulin subclasses.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 167145
- 48409-7
- 48390-9
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
12 mL
Minimum Volume
8 mL
Container
Yellow-top (ACD) tube (solution A or B)
Patient Preparation
None
Storage Instructions
Maintain whole blood at room temperature. Specimens should be analyzed within 72 hours of collection.
Causes for Rejection
EDTA or heparin tube; hemolysis; clotted specimen; donor samples exceeding 72 hours
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 1 week |
| Frozen | Indefinitely |