Complement C3a
Also known as: acylation stimulation factor, Complement C3a des-Arginine
Use
This test is used for the measurement of complement C3a and C3a-desArg levels in serum.
Special Instructions
Futhan preservative must be added to samples immediately after collection. Collection kits with this preservative are available as Labcorp No. 78946. Avoid radioactive isotopes 48 hours prior to venipuncture. Proper collection and storage are crucial to avoid in vitro activation of complement.
Limitations
This test was developed and its performance characteristics determined by Labcorp; it has not been FDA cleared or approved. Results shouldn't be used diagnostically without confirmation from other procedures. Elevated C3a is not predictive of specific diseases, normal pregnancy elevates C3a, and complement activation must be minimized during sample handling to prevent in vitro activation.
Methodology
Immunoassay (RIA)
Biomarkers
LOINC Codes
- 4488-3
- 4488-3
Result Turnaround Time
4-7 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
0.5 mL
Container
Lavender-top (EDTA) tube
Collection Instructions
Collect EDTA whole blood into a chilled lavender-top tube. Add Futhan preservative immediately as directed in the Futhan collection kit (Labcorp No. 78946). Mix by inverting several times. Centrifuge whole blood to separate the plasma and transfer to a plastic screw-cap tube labeled "Futhan Added." Freeze immediately and maintain frozen.
Patient Preparation
No radioactive isotopes administered within 48 hours prior to venipuncture.
Storage Instructions
Freeze.
Causes for Rejection
Any tube without "Futhan Added" label; nonfrozen specimen; non-EDTA plasma sample.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 6 hours |
| Frozen | 14 days |