Hemoglobin (Hb) Solubility
Also known as: Hb S, Hemoglobin S, Hgb Solubility, Sickle Cell Preparation, Sickle Cell Solubility Test
Use
Qualitative determination of presence of hemoglobin S; detect sickling hemoglobins; evaluate hemolytic anemia, undiagnosed hereditary anemia with morphologic (sickle-like) abnormalities on peripheral blood smear
Special Instructions
For accurate results, submit a separate lavender-top tube for each test requiring a lavender-top tube to avoid delays in turnaround time. This test is not suitable for neonates or children under six months of age as hemoglobin S levels do not stabilize until after six months.
Limitations
The Hemoglobin (Hb) Solubility test may yield false-positive results due to polycythemic blood, hyperglobulinemia, or various abnormal hemoglobins such as Hemoglobin I, Bart, CGeorgetown, Alexandra, CHarlem, Porto Alegre, Memphis/S, CZiguinchor, and STravis. Hemoglobin fractionation should confirm positive tests. False-negative results can occur with inadequate blood quantities, high Hb F levels, or certain medications. The presence of hemoglobin S is genetically delayed and may be undetectable in newborns or post-transfusion patients. Solubility tests are unreliable in children younger than six months.
Methodology
Other
Biomarkers
LOINC Codes
- 6864-3
- 6864-3
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.1 mL
Container
Lavender-top (EDTA) tube; capillary puncture: three microhematocrit tubes. May also use blue-top (sodium citrate) tube, yellow-top (ACD) tube, or green-top (heparin) tube.
Collection Instructions
Submit a separate lavender-top tube for this test to avoid TAT delays.
Patient Preparation
No preliminary fluid or food restriction is required.
Storage Instructions
Room temperature.
Causes for Rejection
Specimen other than whole blood received; clotted specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |