Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to HIV-1 GenoSure® MG
Also known as: HIV-1 RNA by Real-time PCR, Quantitative, Reflex to Genotype, Viral Load
Use
Detect and quantitate HIV-1 in plasma with reflex to genotype (at additional charge) if viral load is >500 copies/mL. GenoSure® MG test detects mutations in the HIV viral genes that encode the HIV protease and polymerase (reverse transcriptase). Mutations in these genes have been associated with resistance to antiretroviral drugs.
Special Instructions
Submit the patient's Social Security number for serial monitoring. To order this test without the serial monitor service, use test 550432. Ensure that the plasma specimen is centrifuged within six hours of collection and frozen immediately.
Limitations
The assay range for this test is between 20 and 10,000,000 copies/mL. Specimens must be handled carefully to avoid rejection due to hemolysis or improper container use. The use of green-top (heparin) tubes is not acceptable and may result in rejection. Specimens must be frozen immediately and shipped in a screw-cap polypropylene frozen transport tube.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 20447-9
- 29541-0
- 80563-0
Result Turnaround Time
9-16 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Screw-cap polypropylene frozen transport tube
Collection Instructions
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20° C. Do not place samples on dry ice until completely frozen. Ship specimens frozen.
Storage Instructions
Freeze. Stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; quantity not sufficient for analysis; frozen PPT™
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 5 days |