Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to HIV-1 GenoSure PRIme®
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA Real-time PCR Quantitative, Reflex to GenoSure PRIme®, Viral Load
Use
Detect and measure HIV-1 in plasma with reflex to GenoSure PRIme® if viral load is ≥500 copies/mL. GenoSure PRIme® assay detects mutations in the HIV viral genes that encode for HIV protease, reverse transcriptase and integrase. Mutations in these genes have been associated with resistance to antiretroviral drugs.
Special Instructions
Submit the patient's Social Security number for serial monitoring. To order this test without serial monitoring, use test 550655. Submit a separate frozen specimen for each test requested.
Limitations
The assay range is from 20 to 10,000,000 copies/mL. This test may face limitations such as potential hemolysis, use of incorrect tubes (e.g., green-top heparin), nonfrozen specimens, specimens exposed to repeated freeze/thaw cycles, and insufficient quantity for analysis. Also, the frozen PPT™ tubes may be rejected.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 20447-9
- 20447-9
- 29541-0
- 80563-0
Result Turnaround Time
17-25 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Lavender-top (EDTA) or plasma preparation tubes (PPT™). Ship in two or more screw-cap polypropylene frozen transport tubes.
Collection Instructions
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20°C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze. A separate frozen sample is required for GenoSure PRIme portion of the test. Frozen sample preferred for viral load assay but also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; quantity not sufficient for analysis; frozen PPT™
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 5 days |