Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to PhenoSense®
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time (RT) PCR, Quantitative, Reflex to PhenoSense®, Viral Load
Use
Detect and quantitate HIV-1 in plasma with reflex to phenotype (at additional charge) if viral load is >500 copies/mL. Test determines virus resistance to antiretroviral drugs.
Special Instructions
Reflex to phenotype is performed at an additional charge if the viral load exceeds 500 copies/mL. Proper specimen collection and handling are crucial to ensure correct test results. Specimens should be collected in lavender-top (EDTA) tubes and processed promptly.
Limitations
The assay range for this test is from 20 to 10,000,000 copies/mL. Specimens collected in green-top (heparin) tubes or improper transportation methods can lead to false results. Additionally, repeat testing cannot be performed if the minimum volume requirement is not met.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 20447-9
- 20447-9
- 29541-0
- 80563-0
Result Turnaround Time
17-25 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Ship in two or more screw-cap polypropylene frozen transport tubes.
Collection Instructions
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer to screw-cap tubes, and freeze. Do not use green-top (heparin) tubes.
Storage Instructions
Freeze for long-term storage. Frozen specimens are preferred but stable at room temperature for one day or refrigerated for five days.
Causes for Rejection
Hemolysis; green-top (heparin) tube; specimen received in "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; frozen PPT™
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 5 days |
| Frozen | Indefinitely |