Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to PhenoSense Entry®
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time (RT) PCR, Quantitative, Reflex to PhenoSense Entry®, Viral Load
Use
Detect and quantitate HIV-1 in plasma with reflex to phenotype (at additional charge) if viral load is >1000 copies/mL.
Special Instructions
Not provided.
Limitations
The assay can reliably measure HIV-1 RNA levels within the range of 20 to 10,000,000 copies/mL. Parameters outside this range may affect the test's accuracy. Specimens that do not adhere to collection and storage instructions, such as those collected in green-top tubes or transferred incorrectly, may lead to inaccurate results or test rejection.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 20447-9 - HIV1 RNA # SerPl NAA+probe
- 20447-9 - HIV1 RNA # SerPl NAA+probe
- 29541-0 - HIV1 RNA SerPl NAA+probe-Log#
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Screw-cap polypropylene frozen transport tubes
Collection Instructions
Collect specimen in lavender-top (EDTA) or PPT™ tubes. Centrifuge within six hours of collection. Transfer plasma to screw-cap polypropylene transport tubes and freeze. Ship frozen.
Storage Instructions
Freeze for long-term storage; stable at room temperature for one day or refrigerated for five days.
Causes for Rejection
Hemolysis; green-top (heparin) tube; specimen in 'pop-top' or 'snap-cap' tube; insufficient quantity; frozen PPT™
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 5 days |
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