Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to Trofile®
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time (RT) PCR, Quantitative, Reflex to Trofile®, Viral Load
Use
Detect and quantitate HIV-1 in plasma with reflex to phenotype (at additional charge) if viral load is >1000 copies/mL.
Special Instructions
Not provided.
Limitations
The assay quantifies HIV-1 viral load in plasma within a range of 20 to 10,000,000 copies/mL. It can be affected by the type of tubes used for specimen collection and storage. Green-top (heparin) tubes and 'pop-top' or 'snap-cap' tubes are not suitable as they risk false results or rejection of the sample.
Methodology
PCR-based (Real-time PCR)
Biomarkers
Human Immunodeficiency Virus 1
Microorganism
LOINC Codes
- 20447-9 - HIV1 RNA # SerPl NAA+probe
- 20447-9 - HIV1 RNA # SerPl NAA+probe
- 29541-0 - HIV1 RNA SerPl NAA+probe-Log#
- 51969-4 - Gene analysis narr rpt Doc
- 50790-5 - HIV1 RNA tropism Islt
- 57182-8 - HIV1 RNA tropism Plas
- 77202-0 - Laboratory comment Report
- 49549-9 - Ref lab test method
- 75608-0 - Citation Ref Lab Test
Result Turnaround Time
7-25 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Screw-cap polypropylene frozen transport tubes
Collection Instructions
Collect in three lavender-top (EDTA) tubes or three PPT™ tubes. Centrifuge within six hours. Transfer plasma to screw-cap tubes and freeze. Do not use green-top tubes. Ship frozen.
Storage Instructions
Freeze. Separate frozen sample required for Trofile® portion. Stable at room temperature for one day, refrigerated for five days, and freeze for long-term storage.
Causes for Rejection
Hemolysis, green-top tube, 'pop-top' or 'snap-cap' tube, insufficient quantity, frozen PPT™ tubes.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 5 days |
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