Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to Trofile®

Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time (RT) PCR, Quantitative, Reflex to Trofile®, Viral Load

Clinical Use

Order Test

Use

Detect and quantitate HIV-1 in plasma with reflex to phenotype (at additional charge) if viral load is >1000 copies/mL.

Special Instructions

Not provided.

Limitations

The assay quantifies HIV-1 viral load in plasma within a range of 20 to 10,000,000 copies/mL. It can be affected by the type of tubes used for specimen collection and storage. Green-top (heparin) tubes and 'pop-top' or 'snap-cap' tubes are not suitable as they risk false results or rejection of the sample.

Test Details

Methodology

PCR-based (Real-time PCR)

Biomarkers

Human Immunodeficiency Virus 1

Microorganism

Viral Load

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

log10 HIV-1 RNA

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

LOINC Codes

Order Codes

20447-9 - HIV1 RNA # SerPl NAA+probe

Result Codes

20447-9 - HIV1 RNA # SerPl NAA+probe
29541-0 - HIV1 RNA SerPl NAA+probe-Log#
51969-4 - Gene analysis narr rpt Doc
50790-5 - HIV1 RNA tropism Islt
57182-8 - HIV1 RNA tropism Plas
77202-0 - Laboratory comment Report
49549-9 - Ref lab test method
75608-0 - Citation Ref Lab Test

Result Turnaround Time

7-25 days

Specimen

Plasma

Volume

6 mL

Minimum Volume

4 mL

Container

Screw-cap polypropylene frozen transport tubes

Collection Instructions

Collect in three lavender-top (EDTA) tubes or three PPT™ tubes. Centrifuge within six hours. Transfer plasma to screw-cap tubes and freeze. Do not use green-top tubes. Ship frozen.

Storage Instructions

Freeze. Separate frozen sample required for Trofile® portion. Stable at room temperature for one day, refrigerated for five days, and freeze for long-term storage.