Legionella species Culture
Also known as: Culture, Legionella sp
Use
Isolate and identify Legionella sp
Special Instructions
For successful isolation of Legionella species, it is crucial to avoid contamination with normal flora from the respiratory tract. Only suitable specimens such as transtracheal aspirates, pleural fluid, or other body tissues offer optimal results for culture. Specimens must be transported to the laboratory within 24 hours of collection to maintain viability. Healthcare providers should contact the laboratory for assistance regarding specimen collection and handling.
Limitations
This culture test's sensitivity ranges from 50% to 80%, though its specificity is 100%. Sputum and bronchial aspirates may suffer from bacterial overgrowth, decreasing the test's effectiveness. A direct fluorescent antibody smear without culture can alternatively detect Legionella, and acute and convalescent sera for Legionella antibodies might be required to maximize diagnostic accuracy. However, seroconversion, a critical diagnostic criterion, may take time to manifest, with only 71% detection at 28 days post-onset. Environmental factors in hospitals can also influence transmission.
Methodology
Culture-based
Biomarkers
LOINC Codes
- 88585-5
- 88585-5
Result Turnaround Time
7-16 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
1 mL lung exudate or 1 cc lung biopsy
Minimum Volume
Not provided
Container
Sterile container
Collection Instructions
Contamination with normal flora from respiratory tract should be avoided. Transport to the laboratory within 24 hours of collection.
Storage Instructions
Refrigerate. Maintain specimens at 2°C to 8°C and transport to the laboratory within 24 hours of collection.
Causes for Rejection
Swab specimen; inappropriate specimen transport device; improper labeling; specimen received after prolonged delay (usually more than 72 hours); specimen received in expired transport; environmental water samples such as air conditioning/cooling towers