5 mL sputum (preferred), minimum 3 mL sputum; alternatively 5–10 mL bronchial wash/aspirate, minimum 10 mL bronchial wash or tracheal aspirate

Not provided

sterile leak‑proof container

Early morning deep cough sputum after gargle with water; bronchial wash or tracheal aspirate similarly collected

Patient should gargle with water and cough deeply to collect deep respiratory specimen