HIV‑1 Resistance, Proviral DNA (RTI, PI, Integrase Inhibitors)
Also known as: Archived DNA, Undetected viral load, Cell-associated Viral DNA, Archived resistance, Resistance Analysis
Use
Used to detect archived HIV‑1 drug resistance mutations in patients with virologic suppression or low‑level viremia (e.g., <1,000 copies/mL), when plasma RNA genotypic resistance testing may not be successful. The test captures historical resistance mutations in proviral DNA from host cells and may guide antiretroviral regimen modifications when plasma RNA is undetectable or insufficient.
Special Instructions
Not provided.
Limitations
PCR primers amplify HIV‑1 subtype B and many major non‑B subtypes (A, C, BF, D, AE, AG, H), but uncommon non‑B subtypes or variants may not amplify. Clinical performance and utility are limited; some resistance mutations may be missed, while others may be archived and present without known clinical significance. Proviral DNA should not be considered a comprehensive archive; plasma RNA genotypes remain informative.
Methodology
NGS
Biomarkers
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
2 mL (minimum 0.6 mL)
Minimum Volume
0.6 mL
Container
lavender‑top EDTA tube
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