Direct Antiglobulin Test (DAT) with Reflex to Anti‑C3 and Anti‑IgG
Also known as: Direct Coombs, Coombs Direct
Use
The DAT (Direct Coomb’s test) is positive if red cells have been coated in vivo with immunoglobulin, complement, or both. A positive result may indicate immune‑mediated red cell destruction such as autoimmune hemolytic anemia, a transfusion reaction, or drug‑induced hemolysis.
Special Instructions
If submitting with any other test requiring whole blood, please submit a separate whole blood tube for the DAT.
Limitations
Not provided.
Methodology
Other
Biomarkers
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
1 mL
Container
EDTA lavender or pink‑top tube or ACD‑A or ACD‑B (yellow‑top) tube
Storage Instructions
Refrigerated
Causes for Rejection
Gross hemolysis, grossly lipemic, serum separator tube, clotted specimen, grossly icteric
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant