Aggressive B-Cell Lymphoma Reflex Panel by FISH, Tissue

Also known as: DLBCL_RFLX

Clinical Use

Use

Aid in the diagnosis of aggressive large B-cell lymphoma with intermediate features between Burkitt lymphoma and diffuse large B-cell lymphoma (DLBCL) and confirmation of suspected double-hit lymphoma. The test can detect 8q24 (MYC) translocations and is performed using FISH to assess MYC rearrangement in various B-cell lymphomas, including 'double-hit' or 'triple-hit' lymphomas. The results should be correlated with clinical, morphologic, and immunophenotypic data.

Special Instructions

Not provided.

Limitations

The test is expected to detect 100 percent of MYC rearrangements in patients with MYC-rearranged lymphomas, except for rare instances of cryptic rearrangements. The use of this assay on decalcified tissues has not been validated. Results should be interpreted with caution. Some signal patterns other than the classic abnormal pattern may also be present and may be considered abnormal.

Test Details

New York Approved

Methodology

Chromosomal / Cytogenetics (FISH)

Biomarkers

LOINC Codes

Result Codes

78236-7 - Cells.IGH rear/Cells cnt NFr Bld/T FISH
81304-8 - Struct var analysis method

Result Turnaround Time

3-7 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

4 slides

Container

tissue block or 8 unstained 3-micron slides

Collection Instructions

Formalin fix (10 percent neutral buffered formalin) and paraffin-embed tissue. Transport tissue block or slides in a tissue transport kit.

Storage Instructions

Room temperature. Also acceptable: Refrigerated. Protect paraffin block from excessive heat. Ship in cooled container during summer months.

Causes for Rejection

Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens.