CIC (19q13.2) Gene Rearrangement by FISH
Also known as: CIC FISH
Use
This test is used to diagnose CIC-rearranged sarcoma (CRS) by detecting rearrangements involving the CIC gene. It is essential in diagnosing CIC-rearranged sarcoma, where an abnormal signal pattern in 25% or more of the tumor cells is considered a positive result. CIC rearrangements can also occur in other tumors, such as angiosarcoma and central nervous system tumors. This test aids in the identification of CIC gene rearrangements but does not identify the specific partner gene involved in the rearrangement.
Special Instructions
Include the surgical pathology report with the specimen. If multiple specimens are sent, specify in the order comment which specimen to test or submit individual orders for each sample. A Pathologist Block Selection Fee may apply if ARUP is to choose the specimen.
Limitations
The test does not identify the translocation partner of the CIC gene. While it is designed to detect rearrangements involving the CIC gene, rare cryptic rearrangements may not be detected. The clinical interpretation must correlate with histopathologic and clinical findings for comprehensive analysis.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 81304-8
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 5 unstained (4-micron thick sections) positively charged slides in a tissue transport kit. Min. 2 slides required.
Storage Instructions
Room temperature storage is preferable, refrigerated shipping is acceptable, especially during summer months.
Causes for Rejection
Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |