Glypican 3 by Immunohistochemistry
Also known as: GLYP3 IHC
Use
This test provides immunohistochemical staining for Glypican-3, a protein useful in differentiating hepatocellular carcinoma from other malignancies and hepatic adenomas, as it is expressed in a high proportion of hepatocellular carcinomas; the stain is provided as a technical (stain‑and‑return) service only. It aids pathologists in supporting a diagnosis based on tissue morphology.
Special Instructions
This is performed as a stain‑and‑return technical service only. Ordering recommendations refer to ARUP's Immunohistochemistry Stain Offerings brochure. The tissue should be formalin fixed and paraffin embedded (or cellblock), and either the paraffin block or a minimum of 2 unstained (3‑ to 5‑micron), positively charged slides may be sent. ARUP supply #47808 tissue transport kit is recommended but not required. Slides should not be oven baked if precut. The tissue should be protected from excessive heat. Transport at room temperature (also acceptable refrigerated), with shipping in a cooled container during summer months.
Limitations
Specimens that are submitted with non‑representative tissue type or depleted specimens are unacceptable. Frozen specimens are unacceptable. The test does not include pathologist interpretation—it is technical only. Ambient and refrigerated specimens remain stable indefinitely.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 41098-5
- 57723-9
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Transport tissue block or 5 unstained (3‑ to 5‑micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808 recommended but not required). If sending precut slides, do not oven bake.
Storage Instructions
Transport at room temperature; refrigerated acceptable; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |