H3K27M by Immunohistochemistry
Also known as: H3K27M IHC
Use
This immunohistochemical stain detects the presence of the histone H3 K27M mutation in tissue specimens. The presence of the H3K27M mutation is diagnostic of diffuse midline glioma H3K27M-mutant, a clinically significant entity with prognostic implications, including poorer treatment response and adverse outcomes compared to wild-type tumors ([pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/30647848/?utm_source=openai)).
Special Instructions
Not provided.
Limitations
Specimen must be formalin-fixed, paraffin-embedded tissue or equivalent cell block with adequate representation; depleted specimens or non‑representative tissue types are unacceptable. Slides should be positively charged, 3–5 micron thick; if precut slides are sent, do not oven bake. Stain results are technical only; interpretation must be performed separately. Frozen specimens are unacceptable. Technical stain only — no pathologist interpretation is included ([ltd.aruplab.com](https://ltd.aruplab.com/index.php/Tests/Pub/3004370?utm_source=openai)).
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
H3K27M
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue transport kit (ARUP supply #47808) or paraffin block or 5 unstained positively charged slides (3‑ to 5‑micron thick) (min: 2 slides)
Collection Instructions
Transport tissue block or unstained slides in a tissue transport kit; if sending precut slides, do not oven bake.
Storage Instructions
Room temperature; refrigerated acceptable; ship cooled during summer
Causes for Rejection
Non‑representative tissue type; depleted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
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