H3K27me3 by Immunohistochemistry
Also known as: H3K27M3IHC
Use
Immunohistochemical staining for H3K27me3 (histone H3 lysine 27 trimethylation) is used as a technical stain-and-return service, typically to assist in diagnostic pathology. It is often helpful in evaluating tumor types such as malignant peripheral nerve sheath tumors or other neoplasms where loss or retention of H3K27me3 expression provides diagnostic insight. This test is performed laboratory‑developed test (LDT) and cannot be used for definitive mutation analysis, but serves as an adjunct immunohistochemical marker.
Special Instructions
Not provided.
Limitations
Only technical stain‑and‑return service is offered; interpretive evaluation must be done by the submitting facility. Frozen specimens are unacceptable. Slides or blocks must be represented; depleted or non‑representative tissue is unacceptable. Limited to immunohistochemical detection of H3K27me3 and does not assess other molecular alterations.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Transport tissue block or 5 unstained (3‑ to 5‑micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808). If sending precut slides, do not oven bake.
Storage Instructions
Room temperature; Refrigerated acceptable. Ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; Depleted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant