Lymphocyte Antigen and Mitogen Proliferation Panel
Also known as: LAM
Use
The Lymphocyte Antigen and Mitogen Proliferation Panel is used to evaluate patients with suspected immunodeficiency diseases, including severe combined immunodeficiency. It tests lymphocyte proliferation in response to antigens such as phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen. The test results are presented as counts per minute (CPM) of stimulated versus control lymphocytes and a stimulation index (SI), providing insight into the functional responsiveness of a patient's lymphocytes.
Special Instructions
Patient and control specimens must be collected within 48 hours of test performance. A control specimen from a healthy individual collected under similar conditions is required. For patients who are lymphopenic, collection of a larger blood volume (10 mL) is recommended to increase the likelihood of isolating sufficient lymphocytes.
Limitations
Abnormal results should be interpreted with caution if a client control sample is not submitted, as results may be affected by artifacts during specimen collection, transport, and handling. The test is not suitable for patients younger than 3 months unless there is a clinical history of candidiasis. Specimens that are refrigerated or frozen, or those collected in yellow ACD solution B, are not acceptable.
New York Approved
Methodology
Cell-based / Cytometry
Biomarkers
LOINC Codes
- 59063-8
Result Turnaround Time
9-10 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL (patient) AND 10 mL (control)
Minimum Volume
7 mL (patient) AND 7 mL (control)
Container
Green (sodium heparin) OR Yellow (ACD solution A)
Collection Instructions
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as the patient sample.
Storage Instructions
Transport at room temperature. Do not refrigerate or freeze.
Causes for Rejection
Yellow (ACD solution B), refrigerated or frozen specimens, specimens in transport longer than 48 hours.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |