Pokeweed Mitogen (PWM)‑induced Lymphocyte Proliferation
Also known as: Mitogen-induced Cell Proliferation, Lymphocyte Stimulation
Use
Assessment of cellular immune responsiveness via nonspecific mitogen‑induced lymphocyte proliferation; helps evaluate immune competence by measuring proliferation of T and B lymphocytes in response to pokeweed mitogen (PWM) as a stimulatory agent, often in cases of suspected immunodeficiency or broadly suppressed cellular immunity.
Special Instructions
Test is available by prior arrangement only. In Quest Diagnostics’ Mitogen‑and Antigen‑induced Lymphocyte Proliferation Panel (MITOANT), PWM is one of several stimulants included alongside PHA, Con A, Candida, Tetanus, and tuberculin PPD.
Limitations
Whole blood must be transported promptly at room temperature and processed within 48 hours of collection; specimens that are refrigerated, frozen, hemolyzed, clotted, or collected in lithium heparin tubes are unacceptable. Analytical performance characteristics have been determined by Quest Diagnostics (CLIA‑validated); the assay is not FDA‑cleared.
Methodology
Culture‑based
Biomarkers
Result Turnaround Time
15 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
Not provided
Minimum Volume
Not provided
Container
sodium heparin (green‑top) tube
Collection Instructions
Preferred: 20 mL whole blood in sodium heparin; draw Mon–Thu before noon; send to lab immediately; transport at room temperature within 48 hours.
Causes for Rejection
Hemolysis; refrigerated; frozen; clotted specimens; use of lithium heparin tubes
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |