Lymphocyte Antigen Proliferation

Also known as: LAPP

Clinical Use

Use

The Lymphocyte Antigen Proliferation test is instrumental in evaluating the functionality of lymphocytes in response to specific antigens. This test is crucial for assessing immune competence, particularly in patients with suspected immune deficiencies or dysfunctions. By measuring the proliferation of lymphocytes exposed to candida and tetanus antigens, the test provides valuable insights into the patient's immune response, which can guide clinical management in conditions like immunodeficiency disorders.

Special Instructions

Not provided.

Limitations

The test requires precise collection and transport conditions to avoid artifacts in results. Specimens must remain at ambient temperature and cannot be refrigerated or frozen. The test cannot be performed on specimens collected in ACD solution B and is unacceptable if transported for more than 48 hours. Lymphocyte counts too low could result in inadequate testing conditions.

Test Details

New York Approved

Methodology

Cell-based / Cytometry (Flow Cytometry)

Biomarkers

Candida

Analyte

Proliferation Response

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

Tetanus

Analyte

Proliferation Response

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

Result Turnaround Time

9-10 days

Specimen

Whole Blood

Volume

10 mL (patient) AND 10 mL (control)

Minimum Volume

7 mL (patient) AND 7 mL (control)

Container

Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control).