Lymphocyte Antigen Proliferation
Also known as: LAPP
Use
The Lymphocyte Antigen Proliferation test is instrumental in evaluating the functionality of lymphocytes in response to specific antigens. This test is crucial for assessing immune competence, particularly in patients with suspected immune deficiencies or dysfunctions. By measuring the proliferation of lymphocytes exposed to candida and tetanus antigens, the test provides valuable insights into the patient's immune response, which can guide clinical management in conditions like immunodeficiency disorders.
Special Instructions
Do not order for patients younger than 3 months unless there is a clinical history of candidiasis. Collection of 10 mL of whole blood for lymphopenic patients is recommended to ensure sufficient isolation of lymphocytes. Both patient and control specimens should be collected simultaneously and under similar conditions.
Limitations
The test requires precise collection and transport conditions to avoid artifacts in results. Specimens must remain at ambient temperature and cannot be refrigerated or frozen. The test cannot be performed on specimens collected in ACD solution B and is unacceptable if transported for more than 48 hours. Lymphocyte counts too low could result in inadequate testing conditions.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
Result Turnaround Time
9-10 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL (patient) AND 10 mL (control)
Minimum Volume
7 mL (patient) AND 7 mL (control)
Container
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control).
Collection Instructions
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions.
Storage Instructions
Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Causes for Rejection
Yellow (ACD solution B), refrigerated or frozen specimens, specimens in transport longer than 48 hours.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |