T- and B-Lymphocyte and Natural Killer Cell Profile
Use
The T- and B-Lymphocyte and Natural Killer Cell Profile is a diagnostic test that involves the enumeration of various lymphocyte subsets including T-cells, B-cells, and NK cells. This profile can be particularly significant in monitoring immune function and disease progression in patients, such as those with HIV-1. For AIDS patients, the test provides important prognostic data, particularly CD4 and CD8 T cell levels, which are used to monitor disease progression and retroviral therapy efficacy. Additionally, natural killer cells' activity against virus-infected and malignant cells helps in evaluating the cytotoxic activity of the immune system.
Special Instructions
Not provided.
Limitations
The test results are affected by the stability and collection conditions of the specimen. Incorrect temperatures or delayed transportation might compromise cell viability and immunophenotyping results. Additionally, slight variations in equipment and reagents between laboratories may affect the precision and repeatability of the cell counts.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 65758-5 - T-cell CD4 subset pnl Bld
- 8116-6 - CD19 Cells # Bld
- 8117-4 - CD19 Cells NFr Bld
- 8122-4 - CD3 Cells # Bld
- 8124-0 - CD3 Cells NFr Bld
- 24467-3 - CD3+CD4+ Cells # Bld
- 8123-2 - CD3+CD4+ Cells NFr Bld
- 14135-8 - CD3+CD8+ Cells # Bld
- 8101-8 - CD3+CD8+ Cells NFr Bld
- 54218-3 - CD3+CD4+ Cells/CD3+CD8+ Cll Bld
- 9728-7 - CD3-CD16+CD56+ Cells # Bld
- 8112-5 - CD3-CD16+CD56+ Cells NFr Bld
- 6690-2 - WBC # Bld Auto
- 789-8 - RBC # Bld Auto
- 718-7 - Hgb Bld-mCnc
- 4544-3 - Hct VFr Bld Auto
- 787-2 - MCV RBC Auto
- 785-6 - MCH RBC Qn Auto
- 786-4 - MCHC RBC Auto-EntMCnc
- 788-0 - Erythrocyte DistWidth Bld Auto
- 777-3 - Platelet # Bld Auto
- 770-8 - Neutrophils NFr Bld Auto
- 736-9 - Lymphocytes NFr Bld Auto
- 5905-5 - Monocytes NFr Bld Auto
- 713-8 - Eosinophil NFr Bld Auto
- 706-2 - Basophils NFr Bld Auto
- 75652-8 - Imm Lymphs Bld Ql
- 751-8 - Neutrophils # Bld Auto
- 731-0 - Lymphocytes # Bld Auto
- 742-7 - Monocytes # Bld Auto
- 711-2 - Eosinophil # Bld Auto
- 704-7 - Basophils # Bld Auto
- 71695-1 - Imm Granulocytes NFr Bld Auto
- 53115-2 - Imm Granulocytes # Bld Auto
- 58413-6 - nRBC Bld Auto-Rto
- 18314-5 - Morphology Bld-Imp
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
Fill tube(s) to capacity
Minimum Volume
Not provided
Container
Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube
Collection Instructions
Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Yellow-top (ACD-A) or (ACD-B) tube: 2 days, Lavender-top (EDTA) tube: 1 day |
| Refrigerated | Unstable |
| Frozen | Unstable |
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