Special Stain, Alcian Blue - Periodic Acid-Schiff (PAB)
Also known as: PAB SS
Use
Histochemical stain combining Alcian Blue and Periodic Acid–Schiff (PAB), used to identify mucins and glycoproteins in tissue specimens. This stain is provided via ARUP’s stain-and-return technical service, enabling pathologists to visualize acid mucins (blue) and neutral mucins/glycogen (magenta) in formalin-fixed, paraffin-embedded tissues for diagnostic histopathology.
Special Instructions
Anatomic Pathology Test Request Form (ARUP form #32960) is recommended. Submit via electronic request when possible; if not, use the physical form with ARUP client number. This test is a technical stain-and-return service only; slides or blocks are stained and returned to submitting client.
Limitations
Specimens must contain representative tissue and must not be depleted. Frozen specimens are unacceptable. Slides and blocks must be protected from excessive heat. Slides should be 3–5 µm thick, positively charged; minimum one slide. Frozen specimens are unacceptable.
New York Approved
Methodology
Other
Biomarkers
LOINC Codes
- 10766-4
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
1 slide
Container
tissue transport kit (ARUP supply #47808)
Collection Instructions
Transport tissue block or 2 unstained (3‑ to 5‑micron thick) positively charged slides
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months
Causes for Rejection
Nonrepresentative tissue type; depleted specimens; frozen specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |