Special Stain, Periodic Acid‑Schiff (PAS) with Diastase
Also known as: PAS W D SS
Use
This histochemical special stain (Periodic Acid‑Schiff with Diastase digestion) is utilized in anatomic pathology to digest glycogen from tissue specimens so that PAS-reactive non‑glycogen materials—such as mucopolysaccharides, glycoproteins, glycolipids, mucins or fungal organisms—are selectively highlighted, aiding diagnostic evaluation of these components in FFPE tissues.
Special Instructions
Submit an Anatomic Pathology Test Request Form (ARUP form #32960) for ordering; electronic ordering via ARUP Connect is preferred. This test is performed as a stain‑and‑return (technical service) only.
Limitations
Specimen must be representative tissue or cells processed into formalin-fixed, paraffin‑embedded (FFPE) block or cellblock; heat-damaged specimens must be protected. Frozen specimens are unacceptable. Depleted or non‑representative tissue will be rejected. Reagent limitations make this stain a technical procedure only, and interpretation must occur at the submitting site.
Methodology
Other
Biomarkers
LOINC Codes
- 10800-1
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
1 slide
Collection Instructions
Protect paraffin block and/or slides from excessive heat; submit a tissue block or 2 unstained (3‑ to 5‑micron thick) positively charged slides using ARUP tissue transport kit #47808.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Nonrepresentative tissue type; depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
