Oncology FISH Analysis - BCR/ABL: t(9;22) [CML/ALL/AML]
Use
Oncology FISH Analysis - BCR/ABL t(9;22) is a Fluorescence In Situ Hybridization (FISH) test designed to detect a common chromosomal rearrangement associated with chronic myelogenous leukemia (CML), acute lymphoblastic leukemia (ALL), and acute myeloid leukemia (AML). CML is characterized by increased white blood cells in the bone marrow. This assay can identify the Philadelphia chromosome, found in ~95% of CML cases, and is associated with a poor prognosis in ALL and AML. Thus, proper interpretation must consider relevant clinical and pathological data.
Special Instructions
Following this assay, results are essential for confirming diagnoses of leukemia, especially when cytogenetic tests yield inconclusive results. It is beneficial to monitor treatment responses over time. Considerations must be made regarding clinical context and past patient history. This test serves not just diagnostic purposes but also assists in prognostic assessments. It provides a valuable tool in the management of therapies, aiding clinicians in decision-making processes.
Limitations
The FISH assay identifies rearrangements involving ABL1, ASS1, and BCR loci, but cannot detect translocations with other chromosomes. This assay should not be solely relied upon for diagnosing CML, ALL, or AML and must be complemented with other diagnostic tools to confirm a diagnosis or guide therapy.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow (Fresh)
Volume
3-5 cc
Minimum Volume
2 cc
Container
Sodium Heparin Tube (Green-Top)
Collection Instructions
Draw bone marrow in a Sodium Heparin (green-top) tube(s).
Storage Instructions
Ship at ambient temperature (18-25°C/64-77°F) in an insulated container by overnight courier. Specimens should arrive in the laboratory within 48 hrs of collection. Do not heat or freeze.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |