Oncology FISH Analysis – EGFR Amplification
Use
Oncology FISH Analysis – EGFR Amplification is a Fluorescence In Situ Hybridization (FISH) test designed to detect a common gene amplification in many different cancer types. The presence of EGFR amplification is generally associated with a poor prognosis but may predict response to various EGFR-targeted therapies. Results of this test must always be interpreted in the context of relevant clinical and pathological data and should not be used alone for a diagnosis of disease. Indications for Testing include patients with a clinical or suspected diagnosis of cancer and for inclusion in clinical trials.
Special Instructions
Specimens should be fixed in 10% neutral buffered formalin for at least 6 hours, not exceeding 48 hours. The formalin volume must be at least tenfold the specimen's volume. Avoid strong acid decalcification solutions. Failing to adhere to the fixation time may lead to false negative results.
Limitations
This FISH assay tests for amplification of the EGFR loci. Other abnormalities involving this gene cannot be identified with this assay.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Adequate amounts of tumor
Minimum Volume
Not provided
Container
Cooled container
Collection Instructions
Transport Temperature: sent at ambient temperature (18-25°C/64-77°F). Protect paraffin tissue from excessive heat. Ship in cooled container during summer months.
Storage Instructions
Ship at ambient temperature (18-25°C/64-77°F) in an insulated container by overnight courier. Do not heat or freeze.
Causes for Rejection
No tumor in tissue. Frozen specimens and specimens fixed/processed in alternative fixatives.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Acceptable |
Other tests from different labs that may be relevant