Allo-Crossmatch
Also known as: Flow Crossmatch, HLA Crossmatch, T-Cell and B-Cell Crossmatch
Use
Detection of the presence of antidonor antibodies (HLA) in a potential solid organ recipient, T- and B-cell crossmatch detects the presence of anti-Class I/Class II HLA antibodies in the recipient that would recognize donor-specific antigens. The presence of antibodies against donor HLA antigens is a contraindication for transplantation and could lead to immediate/acute organ rejection.
Special Instructions
Not provided.
Limitations
Although the primary antibodies detected by this assay are to HLA, other non-HLA-specific antibodies could cause a positive T- and/or B-cell crossmatch. This testing only demonstrates antibodies recognizing donor antigens and cannot distinguish clinically relevant antibodies (e.g., complement binding ability) from non-clinical antibodies. Additionally, this assay only detects the IgG antidonor antibodies and does not detect IgM or other immunoglobulin subclasses.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
Unknown protein
Protein
LOINC Codes
- 48409-7 - T Cell XM Bld Donr Ql
- 48390-9 - B Cell XM Bld Donr Ql
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
12 mL
Minimum Volume
8 mL
Container
Yellow-top (ACD) tube (solution A or B)
Patient Preparation
None
Storage Instructions
Maintain whole blood and serum at room temperature. Donor specimens should be analyzed within 72 hours of collection.
Causes for Rejection
EDTA or heparin tube; hemolysis; clotted specimen; donor samples exceeding 72 hours
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 1 week (at 4°C) |
| Frozen | Indefinitely (at -20°C) |
Other tests from different labs that may be relevant