Anti-Neutrophil Cytoplasmic Ab (ANCA) by Indirect Immunofluorescence (IFA) (RDL)
Also known as: ANCA Ab, Anti-Neutrophil Cytoplasmic Ab (ANCA)
Use
Although C-ANCA and P-ANCA are typically of the IgG isotype, C-ANCA are frequently reactive with PR-3. P-ANCA frequently react with MPO. ANCA/MPO antibodies are found in 30% in glomerular basement membrane disease (Wegener's Disease). 88% of patients with vasculitis-associated pulmonary hemorrhage have elevated IgM ANCA, whereas no patients who are IgM ANCA-negative have pulmonary hemorrhage. IgA ANCA is associated with Cholangitis, Kawasaki disease, Cystic fibrosis and Henoch-Schonlein purpura.
Special Instructions
Not provided.
Limitations
The performance characteristics of this test were developed and determined by LabCorp. It has not been cleared or approved by the FDA, which limits its recognition as a diagnostic tool by regulatory authorities. The test is subject to interference from hemolyzed, lipemic, or icteric specimens, and results may be affected by bacterial contamination or non-serum specimen types.
Methodology
Immunoassay (Indirect Immunofluorescence)
Biomarkers
C-ANCA
ProteinP-ANCA
Protein
LOINC Codes
- 35279-9 - ANCA Ab Ser Ql IF
- 35279-9 - ANCA Ab Ser Ql IF
- 14277-8 - c-ANCA Titr Ser IF
- 14278-6 - p-ANCA Titr Ser IF
- 17357-5 - p-ANCA Ser Ql IF
- 42254-3 - ANA Ser Ql IF
- 20398-4 - ANA Homogen Titr Ser
- 20399-2 - ANA nucleolar Titr Ser
- 20401-6 - ANA Speckled Titr Ser
- 53993-2 - ANA centrosomal Titr Ser IF
- 53003-0 - Mitotic spindle app Ab Titr SerPl IF
- 54006-2 - ANA nuclear membrane pores Titr Ser IF
- 54155-7 - ANA intercell bridge Titr Ser IF
- 53998-1 - ANA nuclear dots Titr Ser IF
- 53006-3 - ENA PCNA Ab Titr Ser IF
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.
Storage Instructions
Refrigerate or freeze.
Causes for Rejection
Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 60 days |
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