Anti-Neutrophil Cytoplasmic Ab (ANCA) by Indirect Immunofluorescence (IFA) (RDL)

Casandra

Casandra Test Code LC12307Version 1 (DRAFT)

Performing Lab

Lab LabcorpLab Test ID 520090CPT Code 86036

Anti-Neutrophil Cytoplasmic Ab (ANCA) by Indirect Immunofluorescence (IFA) (RDL)

Also known as: ANCA Ab, Anti-Neutrophil Cytoplasmic Ab (ANCA)

Clinical Use

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Use

Although C-ANCA and P-ANCA are typically of the IgG isotype, C-ANCA are frequently reactive with PR-3. P-ANCA frequently react with MPO. ANCA/MPO antibodies are found in 30% in glomerular basement membrane disease (Wegener's Disease). 88% of patients with vasculitis-associated pulmonary hemorrhage have elevated IgM ANCA, whereas no patients who are IgM ANCA-negative have pulmonary hemorrhage. IgA ANCA is associated with Cholangitis, Kawasaki disease, Cystic fibrosis and Henoch-Schonlein purpura.

Special Instructions

This test reflexes to Anti-Nuclear Ab by IFA (RDL) if ANCA is positive in order to rule out false positive ANCA due to the presence of ANA. Separate serum from cells within one hour of collection and transfer to a plastic transport tube before shipping. To avoid delays, submit separate frozen specimens for each test requested.

Limitations

The performance characteristics of this test were developed and determined by LabCorp. It has not been cleared or approved by the FDA, which limits its recognition as a diagnostic tool by regulatory authorities. The test is subject to interference from hemolyzed, lipemic, or icteric specimens, and results may be affected by bacterial contamination or non-serum specimen types.

Test Details