Bilirubin, Total and Direct
Also known as: Bilirubin, Total, Conjugated, and Unconjugated
Use
Liver and biliary tests are useful in the differential diagnosis of jaundice from bilirubin overproduction (hemolysis), decreased uptake (Gilbert disease), decreased conjugation (hepatocellular disease, familial, drug-induced, pregnancy; obstructive bile duct disease).
Special Instructions
Separate serum or plasma from cells within 45 minutes of collection. The test is useful for identifying jaundice causes, making it critical to follow specific collection and processing instructions to ensure accurate results. Improper labeling and severe hemolysis or lipemia can affect the test outcomes.
Limitations
The accuracy of this test may be impacted by specimen handling and processing times. Severe hemolysis, improper labeling, or severe lipemia can render the specimen unsuitable for analysis. Additionally, variations in testing schedules or requirements for additional confirmatory tests may affect result turnaround time.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 34543-9
- 1975-2
- 1968-7
- 1971-1
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
3 mL
Minimum Volume
1 mL
Container
Red-top tube, gel-barrier tube, or green-top (lithium heparin) tube
Collection Instructions
Separate serum or plasma from cells within 45 minutes of collection.
Storage Instructions
Refrigerate
Causes for Rejection
Severe hemolysis; improper labeling; severe lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 days |
| Refrigerated | 3 days |
| Frozen | 14 days |