Cortisone, LC/MS-MS (Endocrine Sciences)
Also known as: Compound E
Use
The mineralocorticoid receptor binds cortisol as an active ligand, yet in normal people cortisol levels are not related to sodium retention. While in vitro the mineralocorticoid receptor has a similar affinity as aldosterone and cortisol, and in vivo cortisol is found in concentrations 100 to 1000 times higher than those of aldosterone, 11β-hydroxysteroid dehydrogenase 2 protects the mineralocorticoid receptor by reducing cortisol to cortisone, which is not an active ligand. This enzyme occurs in cells that contain the receptor. A similar enzyme is present in other tissues but works more slowly and backwards, converting cortisone to cortisol. Defects in this system can cause hypertension and can be diagnosed from the cortisol to cortisone ratio.1-6
Special Instructions
Not provided.
Limitations
Results are for investigational purposes only and should not be used as a stand-alone diagnostic procedure. Confirmation of the diagnosis by another medically established diagnostic product or procedure is recommended. The performance characteristics of this assay have been determined by LabCorp.
Methodology
Mass Spectrometry
Biomarkers
Cortisone
Analyte
LOINC Codes
- 14159-8 - Cortisone SerPl-mCnc
- 14159-8 - Cortisone SerPl-mCnc
Result Turnaround Time
4-10 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.2 mL
Container
Gel-barrier tube, red-top tube, lavender-top (EDTA) tube, or green-top (heparin) tube
Collection Instructions
Serum/plasma must be separated from cells within four hours of venipuncture. Send serum/plasma in a plastic transport tube.
Storage Instructions
Freeze (preferred) or refrigerate
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | 7 days |
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