25‑Hydroxyvitamin D, Immunoassay
Also known as: 25 OH Vitamin D, Vitamin D, 25-OH, 25-Hydroxy Vitamin D, Vitamin D
Use
Measurement of total 25‑hydroxyvitamin D [25(OH)D] concentrations in serum provides a good index of circulating vitamin D activity. It is used to screen patients not on vitamin D₂ supplementation for vitamin D deficiency or insufficiency, which can lead to bone diseases such as rickets and osteomalacia, or complications like hypercalcemia. Ideal ranges (30–100 ng/mL) indicate optimal status, below suggests deficiency or insufficiency, and above may reflect toxicity.
Special Instructions
Not provided.
Limitations
This immunoassay does not differentiate or measure individual 25‑hydroxyvitamin D₂ and D₃ isomers, nor does it detect or correct for the C3‑epimer form. It may overestimate total vitamin D in patients with D₂ supplementation or in infants with significant C3‑epimer levels, for which LC‑MS/MS methods are preferred.
Methodology
Immunoassay
Biomarkers
25‑Hydroxyvitamin D (total)
Analyte
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
Not provided
Minimum Volume
Not provided
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