CD23, IHC with Interpretation
Use
CD23 is a dendritic cell marker and acts as a B‑cell growth and activation factor. It is strongly expressed on B cells and monocytes, including robust expression on Epstein‑Barr virus–transformed B lymphoblasts. This marker is particularly useful in identifying B‑CLL (B‑cell chronic lymphocytic leukemia/lymphoma), mediastinal large B‑cell lymphoma, and lymphoplasmacytic lymphoma, and may aid in differentiating B‑CLL from mantle cell lymphoma or MALT lymphoma.
Special Instructions
Ideal fixation time for all immunohistochemistry & biopsy specimens is 6‑72 hours in 10 % neutral buffered formalin per CAP guidelines. Slides or tissue block should be transported in IHC specimen transport kit or slide folder, at room temperature.
Limitations
Not provided.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
IHC specimen transport kit or slide folder
Collection Instructions
10 % neutral buffered formalin‑fixed, paraffin‑embedded tissue block, 4‑micron cut, or 2 unstained sections on positively charged slides
Storage Instructions
Room temperature
Causes for Rejection
Received frozen, broken slides, insufficient tumor‑containing block
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | Unacceptable |