QuestAssureD™ 25‑Hydroxyvitamin D (D2, D3), LC/MS/MS

Casandra

Casandra Test Code QD26681Version 1 (DRAFT)

Performing Lab

Lab Quest DiagnosticsLab Test ID 92888

QuestAssureD™ 25‑Hydroxyvitamin D (D2, D3), LC/MS/MS

Also known as: Vitamin D3, 25-hydroxy, D2 Vitamin, D3 Vitamin, 25-Hydroxycholecalciferol (25OHD3)

Clinical Use

Order Test

Use

This assay provides measurement of total 25‑hydroxyvitamin D concentrations including separate quantitation of the D2 and D3 vitamers. It is specifically recommended for patients aged 3 years and older on vitamin D2 supplementation or for situations where differentiation between vitamin D2 and vitamin D3 is clinically relevant, such as assessing contributions of supplementation or nutritional sources.

Special Instructions

Not provided.

Limitations

Although the assay differentiates 25‑hydroxyvitamin D2 and D3, reference and interpretative ranges have not been established for the individual D2 and D3 components of total 25‑hydroxyvitamin D. Interpretation should rely on total 25(OH)D reference ranges.

Test Details

Methodology

Mass Spectrometry

Biomarkers

Vitamin D, 25‑OH, D2

Analyte

Quantitation of 25‑OH‑D2 • Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

Vitamin D, 25‑OH, D3

Analyte

Quantitation of 25‑OH‑D3 • Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

Vitamin D, 25‑OH, Total

Analyte

Quantitation of total 25‑OH‑D • Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

Result Turnaround Time

2-7 days

Specimen

Serum

Volume

Not provided

Minimum Volume

Not provided

Order Test

Casandra

Casandra Test Code QD26681Version 1 (DRAFT)

Performing Lab

Lab Quest DiagnosticsLab Test ID 92888