CD138 (Syndecan-1) by Immunohistochemistry
Also known as: CD138 IHC
Use
CD138, also known as Syndecan-1, is a pivotal biomarker used in the evaluation and diagnosis of hematologic malignancies, including multiple myeloma and other plasma cell neoplasms. The presence of CD138 in plasma cells aids in the identification of these cells within bone marrow biopsies, providing valuable information in the pathological assessment of bone marrow associated disorders. Immunohistochemical staining for CD138 is commonly performed to elucidate the plasma cell population in tissue samples, facilitating the diagnosis and treatment planning for patients with plasma cell dyscrasias.
Special Instructions
For ordering, submit an electronic request; if unavailable, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. Paraffin block or positively charged slides should be transported using a tissue transport kit (ARUP supply #47808), obtainable via ARUP Connect™ or ARUP Client Services. Ensure specimens are kept away from excessive heat during transport. Consult ARUP Client Services for technical assistance.
Limitations
The CD138 by IHC test is conducted as a stain and return (technical) service only, focusing on tissue or cell samples that have undergone formalin fixation and paraffin embedding. It is crucial that specimens are not depleted and contain representative tissue types to avoid issues with test validity or rejection. The test does not provide comprehensive molecular characterization of plasma cell neoplasms and should be interpreted in accordance with additional diagnostic findings for accurate clinical decision-making.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Paraffin block or 5 unstained (3- to 5-micron thick sections), positively charged slides
Collection Instructions
Formalin fix (10% neutral buffered formalin) and paraffin embed specimen. For cells, prepare into a cellblock. Transport in a cooled container during summer months.
Storage Instructions
Protect paraffin block and/or slides from excessive heat. Ship slides in room temperature or refrigerated conditions.
Causes for Rejection
Depleted specimens. Specimens submitted with non-representative tissue type.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Indefinitely |