CD45 by Immunohistochemistry
Also known as: CD45 IHC
Use
CD45 by immunohistochemistry (IHC), also known as leukocyte common antigen (LCA), is used to detect hematolymphoid lineage cells and distinguish lymphoma/leukemia from other neoplasms. It is highly sensitive and specific for hematolymphoid cells, and is performed as a technical ‘stain and return’ service. Interpretation by a pathologist is not included in this test. Clinical correlation with patient history and additional testing is recommended.
Special Instructions
This is a stain-and-return technical service only. Submit via electronic request or use ARUP Immunohistochemistry Stain Form (#32978) if necessary. Specimen must be formalin-fixed, paraffin-embedded tissue or cell block, transported in ARUP tissue transport kit #47808 (min. 2 slides). Do not oven bake precut slides. Protect from excessive heat. Store at room temperature or refrigerated; use cooled container in summer. Unstained slides (3–5 μm, positively charged) or blocks acceptable. Ambient, refrigerated, and frozen stability: indefinitely. Depleted or non-representative specimens unacceptable. New York State Dept. of Health approval confirmed.
Limitations
Technical service only; pathologist interpretation is not provided and must be ordered separately. Specimens that are depleted or non-representative will be rejected. Oven-baked slides are unacceptable. Cells must be prepared in a cell block if not tissue. Method is limited to detection of CD45 by IHC; cannot detect other markers or diagnoses. Interpretation must consider clinical context.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
ARUP tissue transport kit #47808 (or unstained slides in slide mailer)
Collection Instructions
Formalin fix (10% neutral buffered formalin), paraffin embed specimen. Transport tissue block or min. 2 unstained positively charged slides (3–5 μm thick). Do not oven bake slides.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Depleted specimens; non-representative tissue type; oven-baked slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Indefinitely |