CD43, IHC with Interpretation
Use
CD43 is a cluster of differentiation antigen expressed on T lymphocytes, myeloid cells (including granulocytes and precursors, monocytes, macrophages, histiocytes), plasma cells, and megakaryocytes; immunohistochemical evaluation with interpretation helps in characterizing hematopoietic and related cell types.
Special Instructions
Not provided.
Limitations
Use of fixatives other than 10% formalin may compromise staining quality. FFPE slide age, improper fixation or handling can affect immunoreactivity; charged slide storage and surface chemistry are important for consistent staining.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Minimum of 2 unstained slides (5 µm) or tissue block (~0.2 cm × 0.2 cm × 0.2 cm)
Collection Instructions
FFPE tissue block with corresponding H&E and/or unstained 5 µm slides; send all slides within 6 weeks of cutting; mount on poly‑L‑lysine or charged (+) slides, air dry; do not oven dry.
Storage Instructions
Store at room temperature.
Causes for Rejection
Frozen specimens or poorly fixed tissue; slides older than 6 weeks; improper slide mounting (non‑charged) or drying.
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | Unacceptable |
Other tests from different labs that may be relevant