Celiac Disease Reflexive Cascade, Serum
Also known as: CELIACRFLX
Use
Presence of the tissue transglutaminase (tTG) IgA antibody is associated with gluten-sensitive enteropathies such as celiac disease and dermatitis herpetiformis. Individuals with positive results should be confirmed with small intestinal biopsy to establish celiac disease diagnosis. tTG IgA antibody concentrations greater than 50 FLU exhibit higher correlation with results of duodenal biopsies consistent with celiac disease. For antibody concentrations greater than or equal to 5 FLU but less than 10 FLU, additional testing for endomysial (EMA) IgA concentrations may improve the positive predictive value for disease. A decrease in tTG IgA antibody concentration after initiation of a gluten-free diet may indicate a response to therapy.
Special Instructions
For testing suspected DH, use in conjunction with Immunobullous Disease Antibody Panel (3001409) is recommended. All serologic tests used to diagnose CD should be performed while the patient is on a gluten-containing diet.
Limitations
In individuals who produce sufficient IgA, the most sensitive and specific serologic test for celiac disease (CD) diagnosis is tissue transglutaminase (tTG) IgA. In individuals who are IgA deficient, tTG IgG and deamidated gliadin peptide (DGP) IgG antibody testing is recommended. This reflexive panel test begins by assessing the presence of immunoglobulin A (IgA) using internal control beads. This assay does not measure IgA but flags samples when deficient for IgA.
FDA ApprovedNew York Approved
Methodology
Other
Biomarkers
LOINC Codes
- 46128-5
Result Turnaround Time
2-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
3 mL
Minimum Volume
1.5 mL
Container
ARUP standard transport tube
Collection Instructions
Separate serum from cells ASAP or within 2 hours of collection.
Storage Instructions
Refrigerated
Causes for Rejection
Contaminated, grossly hemolyzed, grossly icteric, or grossly lipemic.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 1 week |
| Frozen | 30 days |