Cytokeratin 7 (CK 7) by Immunohistochemistry
Also known as: CYTO 7 IHC
Use
Cytokeratin 7 (CK 7) immunohistochemistry is used as a qualitative stain to assess CK 7 protein expression in tissue or cellblock specimens. It aids in diagnostic work‑ups of epithelial neoplasms, particularly to help differentiate tumors based on patterns of cytokeratin expression, and serves as a stain‑and‑return technical service on formalin‑fixed, paraffin‑embedded specimens.
Special Instructions
Submit electronic request for this stain; if electronic ordering is unavailable, use ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. Refer to the ARUP Immunohistochemistry Stain Offerings brochure for ordering details. The test is performed as a stain‑and‑return (technical) service only.
Limitations
The test requires representative tissue/cell specimens; non‑representative or depleted specimens are unacceptable. Frozen specimens are unacceptable. Slides should not be oven‑baked. The stain is qualitative and interpretation depends on quality of fixation and preparation; technical and interpretive limitations inherent to immunohistochemistry apply.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue block or 5 unstained (3‑ to 5‑micron thick) positively charged slides in tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. If sending precut slides, do not oven bake.
Storage Instructions
Protect from excessive heat during transport; room temperature or refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens; frozen specimens; oven‑baked slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |