Glucose-6-Phosphate Dehydrogenase
Also known as: G6PD
Use
The Glucose-6-Phosphate Dehydrogenase test is a preferred initial screening for G6PD deficiency. It is essential in diagnosing hemolytic anemia and is particularly significant for patients with certain ethnic backgrounds, such as individuals of African descent, or others at high-risk for G6PD deficiency. The enzyme's activity assessment is crucial for identifying G6PD deficient individuals, which is vital for preventing drug-induced hemolysis and other complications.
Special Instructions
ARUP G6PD results are normalized to Hemoglobin and reported in U/g Hb. Alternative methods may normalize results to red blood cells. Patients who recently received transfusions might show normal results due to donor cells masking deficient erythrocytes.
Limitations
Cutoffs and results for G6PD activity provided by this assay cannot be used interchangeably with other assays due to specific configurations. Males with activity less than 30% and females with activity less than 30% are considered deficient, while females with 80% or more are normal. Intermediate results are for activities between 30%-80% for females. G6PD activity can be falsely normal post-transfusion.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 32546-4
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
1.5 mL
Container
Yellow (ACD solution A). Also acceptable: Green (sodium or lithium heparin), lavender (K2EDTA or K3EDTA), or pink (K2EDTA).
Collection Instructions
Enzyme most stable in acid citrate dextrose (ACD). Do not freeze. ACD collection tubes should be filled to maximum collectible volume and are not recommended for pediatric specimen collection or preservation.
Storage Instructions
Refrigerated
Causes for Rejection
Clotted, frozen, or hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 8 hours |
| Refrigerated | 1 week |
| Frozen | Unacceptable |