Kell K/k (KEL) Antigen Genotyping, Fetal
Also known as: KELGENO FE
Use
The Kell K/k (KEL) Antigen Genotyping, Fetal test is used to assess the risk of alloimmune hemolytic disease of the fetus and newborn (HDFN) by determining the fetal Kell genotype. K antigen is a frequent cause of HDFN and is crucial in erythrocyte alloimmunization, which can lead to hemolytic transfusion reactions or HDFN. Identifying the fetal genotype can help in clinical management and predicting risk in cases where the pregnant patient is sensitized to the big K antigen.
Special Instructions
Not provided.
Limitations
The test may not detect rare nucleotide changes leading to altered or partial antigen expression and null phenotypes. Bloody amniotic fluid samples may result in false-negative outcomes due to maternal cell contamination. Patients who have undergone hematopoietic stem cell transplants might have inconclusive results. The performance of this test has not been cleared or approved by the FDA, and abnormal signal intensities may lead to indeterminate genotyping results.
New York Approved
Methodology
PCR-based (Fragment Analysis)
Biomarkers
No genes
Gene
Result Turnaround Time
3-10 days
Related Documents
For more information, please review the documents below
Specimen
Amniotic Fluid
Volume
10 mL
Minimum Volume
5 mL
Container
sterile container
Collection Instructions
Transport 10 mL amniotic fluid in a sterile container. Backup cultures must be retained at the client's institution until testing is complete.
Storage Instructions
CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to lability of cells.
Causes for Rejection
Frozen specimens in glass collection tubes.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 48 hours |
| Frozen | Unacceptable |
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