Leukemia/Lymphoma Phenotyping Evaluation by Flow Cytometry
Also known as: LL PANEL
Use
Aids in the evaluation of hematopoietic neoplasms such as leukemia and lymphoma by monitoring therapy in patients with an established diagnosis. The test assists in lineage assignment of acute leukemias and aids in the diagnosis and classification of leukemias and lymphomas. Flow cytometry immunophenotyping is utilized to identify and enumerate the different types of cells present in the sample and detect abnormal cell populations indicative of malignancies.
Special Instructions
Specimens must be received at the performing laboratory within 48 hours of collection, particularly due to the short stability of specimens. It is critical to supply complete clinical and diagnostic information to ensure proper marker evaluation. If a previous leukemia/lymphoma phenotyping was performed at a different laboratory, the flow cytometry report and histograms should accompany the specimen. A complete blood count (CBC) and Wright stained smear (if available) should also be provided alongside necessary clinical history and differential diagnosis.
Limitations
This test is not FDA approved but is developed and its performance characteristics determined by ARUP Laboratories for clinical purposes. Immunophenotyping might require correlation with other diagnostic modalities like cytogenetics or morphological analyses to reach a conclusive diagnosis. Results must be interpreted in the context of clinical and laboratory findings, including histopathological correlation for comprehensive diagnosis.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 19099-1
- 31208-2
- 45267-2
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
1 mL
Minimum Volume
0.5 mL
Container
Heparinized container
Collection Instructions
Transport 1 mL heparinized bone marrow, ensure viability by receiving within 24 hours.
Storage Instructions
Room temperature; acceptable if refrigerated.
Causes for Rejection
Clotted or hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 48 hours |
| Frozen | Unacceptable |