MITF by Immunohistochemistry
Also known as: MITF IHC
Use
The MITF by Immunohistochemistry test is utilized to detect the expression of the microphthalmia-associated transcription factor (MITF) in tissue samples. MITF is important for the development and function of melanocytes, the cells responsible for pigmentation in the skin, eyes, and hair. This test is often employed in the differential diagnosis of certain types of melanoma and other soft tissue tumors, providing valuable information that can aid in the assessment and classification of pigmented lesions.
Special Instructions
Not provided.
Limitations
The test is a laboratory-developed test (LDT), which means it was developed and its performance characteristics were determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration (FDA). As with other immunohistochemistry tests, quality of results can be affected by factors such as fixation quality, antigen retrieval, and endogenous enzyme activities, which might lead to false-negative or false-positive outcomes. This test is intended for clinical purposes under CLIA certification but may lack standardized FDA performance validation.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
MITF
Protein
LOINC Codes
- 8251-1 - Service Cmnt-Imp
- 57723-9 - Unique bar code # Current sample
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Tissue block or 5 unstained (3-5 micron thick sections), positively charged slides
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat.
Storage Instructions
Transport at room temperature. Also acceptable refrigerated; ship in cooled container during summer.
Causes for Rejection
Non-representative tissue type or depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant