Myogenin (Myf4) by Immunohistochemistry

Also known as: MYF4 IHC

Clinical Use

Use

The Myogenin (Myf4) by Immunohistochemistry test is used to identify the expression of myogenin in tissues, which is significant in the diagnosis and differentiation of rhabdomyosarcoma from other small round cell tumors. Myogenin is a transcription factor critical for muscle differentiation, and its presence or absence can aid pathologists in determining the muscle origin of a tumor and in differentiating between myogenic and non-myogenic tumors, thus providing crucial information for patient diagnosis and treatment planning.

Special Instructions

Not provided.

Limitations

The test is limited to technical staining procedures only and does not include an interpretive pathology report, which should be completed by the client's pathologist. The effectiveness of the stain is contingent upon the quality of the specimen provided, such as proper fixation and embedding. Inadequate fixation or inappropriate specimen types can lead to suboptimal results.

Test Details

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

Myf4

Protein

IHC

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

LOINC Codes

Result Codes

94736-6 - Tissue block ID Spec

Result Turnaround Time

1-3 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

2 slides

Collection Instructions

Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808).