Pyruvate Kinase
Also known as: PK
Use
The Pyruvate Kinase test is the preferred initial screening method for pyruvate kinase deficiency, which is one of the causes of hemolytic anemia. This condition affects the red blood cells and can lead to premature destruction, affecting the patient's overall health. By quantifying the enzyme pyruvate kinase in red blood cells, this test can provide critical information for the diagnosis and management of hemolytic anemia.
Special Instructions
The stability of the specimen is contingent upon maintaining it in refrigerated conditions. It is important to avoid freezing the specimen as it compromises the integrity of the sample. The test should not be ordered if the patient has recently received a transfusion, as normal donor cells may mask the presence of pyruvate kinase deficient erythrocytes.
Limitations
Patients who have received recent blood transfusions may have normal donor red blood cells that can obscure the presence of pyruvate kinase deficient erythrocytes, possibly leading to inaccurate test results. Additionally, improper specimen storage, such as freezing, can render the test unusable.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 32552-2
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.5 mL
Container
Lavender (EDTA), Pink (K2EDTA), Green (Heparin), Yellow (ACD)
Storage Instructions
Refrigerated
Causes for Rejection
Freezing
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 20 days |
| Frozen | Unacceptable |