S-100 Protein by Immunohistochemistry (DAB Detection)
Also known as: S100 IHC
Use
This test provides a technical service-only immunohistochemical stain (DAB detection) for the S‑100 protein, a marker commonly expressed in Schwann cells, melanocytes, chondrocytes, neural tumors, Langerhans cells, and some carcinomas (e.g., breast). It aids in the identification and characterization of such neoplasms through tissue-specific S‑100 expression patterns.
Special Instructions
Submit electronically or use ARUP Immunohistochemistry Stain Form (#32978) if no electronic ordering capability; requires ARUP client number. Transport tissue block or at least 2 positively charged slides (3–5 µm sections) without oven baking; formalin fixation and paraffin embedding required. Ship in cooled container during summer months.
Limitations
This is a stain‑and‑return (technical component only) service; no pathologist interpretation is provided. The test cannot be used to order components directly. Frozen or improperly fixed tissues are unacceptable; depleted or non‑representative specimens are unacceptable. Slides must avoid excessive heat and be freshly cut.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue block or unstained (3‑5 µm) sections on positively charged slides (min: 2 slides)
Collection Instructions
Formalin fix (10% neutral buffered formalin preferred); paraffin embed; cells must be prepared into a cellblock if cells. Protect paraffin from excessive heat; do not oven bake precut slides.
Storage Instructions
Transport at room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |