Oncology FISH Analysis - MET Amplification

Clinical Use

Use

Oncology FISH Analysis – MET Amplification is designed to detect MET gene amplification, which is commonly associated with non-small cell lung cancer (NSCLC). As the most prevalent lung cancer type, NSCLC often exhibits symptoms like coughing and fatigue that may resemble those of less severe conditions. The identification of MET amplification has significant implications as it generally correlates with a poor prognosis. However, it also serves as a predictive marker for responsiveness to targeted MET therapies. Proper interpretation of the test results requires integration with clinical and pathological information; it should not be relied upon solely for diagnosing NSCLC.

Special Instructions

Specimens for FISH testing should be fixed in 10% neutral buffered formalin for at least 6 hours but no longer than 48 hours. The volume of formalin must be at least 10 times that of the specimen. Avoid using decalcification solutions with strong acids. Specimens fixed incorrectly may yield false negative results.

Limitations

This assay is specifically focused on amplifying the MET loci, therefore it is not equipped to detect other mutations or abnormalities associated with this gene. Thus, any additional genetic anomalies will remain unidentified with this assay.

Test Details